Fri, Oct 26, 2018 @ 11:00 AM - 12:00 PM
Conferences, Lectures, & Seminars
Speaker: Leo Q. Wan, Ph.D., Associate Professor Department of Biomedical Engineering Rensselaer Polytechnic Institute, Troy, NY
Talk Title: Cellular Asymmetry in Development and Disease
Abstract: Cell Chirality in Development and Disease: Cell chirality (also known as handedness and left-right (LR) asymmetry) is an intrinsic capability of the cell telling left from right. The development of the vertebrate body plan with left-right asymmetry requires the emerging chiral morphogenesis at multicellular levels at specific embryonic stages. Changes in orientation of the LR axis due to genetic or environmental factors can lead to malformations and disease. However, the concept of cell chirality has been recognized, but never studied in detail until the recent development of novel engineering tools. In my lab, we demonstrate that the cultivation of cells on micropatterned 2D surfaces and in 3D graded hydrogels reveals an intrinsic cellular LR asymmetry, which is dependent of cell phenotype and actin cytoskeleton. With these new tools, we examine the role of cell chirality on the development of cardiac LR asymmetry as well as the barrier function of endothelium layers. We find that Protein Kinase C (PKC) activation reverses the inherent chirality from clockwise to counter clockwise in engineering systems. Interestingly, activation of PKC signaling reverses the directional bias of chick cardiac C-looping. Mediating endothelial cell chirality can regulate the permeability of endothelial layers. Overall, our results strongly suggest critical roles of cell chirality in cardiovascular development and disease.
Biography: Dr. Leo Q. Wan is an associate professor in the Department of Biomedical Engineering at the Rensselaer Polytechnic Institute in Troy, NY. His research interests focus on understanding physical biology in tissue development and regeneration, and include Tissue Morphogenesis, Stem Cell Mechanobiology, and Functional Tissue Engineering. He received his Bachelor and Master degrees in Mechanical Engineering from the University of Science and Technology of China. After completing his PhD in Biomedical Engineering at Columbia University in 2007, he became a postdoctoral scientist in the area of Stem Cells and Tissue Engineering. Leo is a Pew scholar (Class 2013), and a recipient of the NIH Director's New Innovator Award, National Science Foundation Early Career Award, American Heart Association Scientist Development Grant, and the March of Dimes Basil O'Connor Starter Scholar Research Award.
Host: Keyue Shen, PhD
More Information: Leo Wan Flier.pdf
Audiences: Everyone Is Invited
Contact: Mischalgrace Diasanta